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Valorization of Watermelon (Citrullus lanatus) into Bioethanol Using Several Yeast Strains of Saccharomyces Cerevisiae

Received: 14 December 2023    Accepted: 12 January 2024    Published: 18 January 2024
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Abstract

Watermelon is a fruit with very high losses, and its waste can attract pests. It’s therefore preferable to look for ways to recycle this waste. This study aims to valorize watermelon waste for the production of bioethanol by fermentation. The performance of six yeast strains of Saccharomyces Cerevisiae namely “Zymaflore X16, Actiflore F5, Zymaflore X5, Actiflore BO213, Zymaflore Delta, Zymaflore RX60 and Saf-Levure” were tested, in the presence and absence of urea as a growth factor. The results show that it is possible to obtain ethanol from watermelon waste. Among the six yeast strains studied, four see their fermentation performance improved in the presence of urea. The best yields are obtained for “X5 and RX60” strains with yields close to 18% (compared to less than 4,5% in the absence of urea for the same yeast strains). Nitrogen compounds are therefore nutrients that improve the fermenting power of yeast. The purity of ethanol obtained after distillation of fermentation must varies between 10 and 40% in the absence of urea and between 18 and 42% in the presence of urea. The watermelon from Togo therefore gives interesting and encouraging results for the production of bioethanol with yeast strains that stand out and whose use can be considered for the production of bioethanol on a larger scale. The results obtained will still require optimizing the conditions.

Published in American Journal of Applied Chemistry (Volume 12, Issue 1)
DOI 10.11648/j.ajac.20241201.12
Page(s) 15-21
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Watermelon, Bioethanol, Yeast Strains, Urea

References
[1] Solarte-Toro J. C., Alzate C. A. C. (2021). Biorefineries as the base for accomplishing the sustainable development goals (SDGs) and the transition to bioeconomy: technical aspects, challenges and perspectives, Bioresour. Technol, 340, 125626. https://doi.org/10.1016/j.biortech.2021.125626
[2] Ubando A. T., Felix C. B., Chen W.-H. (2020). Biorefineries in circular bioeconomy: a comprehensive review, Bioresour. Technol, 299, 122585. https://doi.org/10.1016/j.biortech.2019.122585
[3] Azhar S. H. M., Abdulla R., Jambo S. A., Marbawi H., Gansau J. A., Faik A. A. M., and Rodrigues K. F. (2017). Yeasts in sustainable bioethanol production: A review. Biochemistry and biophysics reports, 10, 52-61. https://doi.org/10.1016/j.bbrep.2017.03.003
[4] Soeder D. J. (2021). Fossil Fuels and Climate Change. In: Fracking and the Environment. Springer, Cham. https://doi.org/10.1007/978-3-030-59121-2_9
[5] Mabee, W. E. (2007). Policy options to support biofuel production. Biofuels, 329-357. https://doi.org/10.1007/10_2007_059
[6] Farrell A. E., Plevin R. J., Turner B. T., Jones A. D., O'Hare M., Kammen D. M. (2006). Ethanol can contribute to energy and environmental goals. Science, 311, 506-508. DOI: 10.1126/science.1121416.
[7] Wyman C. (1996). Handbook on bioethanol: production and utilization. CRC Press.
[8] FAO. (2011). Global Food Losses and Food Waste – Extent, Causes and Prevention, Rome, Food and Agriculture Organization of the United Nation.
[9] FAO. (2013). Report of the Expert Consultation Meeting on Food Losses and Waste Reduction in the Near East Region: Towards a Regional Comprehensive Strategy, Cairo, Food and Agriculture Organization of the United Nation.
[10] FAO. (2015). Regional Strategic Framework - Reducing Food Losses and Waste in the Near East and North Africa Region. Cairo, Food and Agriculture Organization of the United Nation.
[11] ONU. (2021). International Year of Fruits and Vegetables. United Nations Organization.
[12] Evcan E., Tari C. (2015). Production of bioethanol from apple pomace by using cocultures: conversion of agro-industrial waste to value added product, Energy, 88, 775–782. https://doi.org/10.1016/j.energy.2015.05.090
[13] Hegde S., Lodge J. S., Trabold T. A. (2018). Characteristics of food processing wastes and their use in sustainable alcohol production, Renewable and Sustainable Energy Review, 81 510–523. https://doi.org/10.1016/j.rser.2017.07.012
[14] Scapini T., Favaretto D. P. C., Camargo A. F., Czapela F. F., Bonatto C., Zanivan J., Dalastra C., Klanovicz N., Souza T., Muller C., Fongaro G., Treichel H. (2019). Bioethanol from fruit. Ethanol as a Green Alternative Fuel: Insight and Perspectives, Nova Science Publishers, New York, 139–176.
[15] Zhao C., Yan H., Liu Y., Huang Y., Zhang R., Chen C., Liu G. (2016). Bio-energy conversion performance, biodegradability, and kinetic analysis of different fruit residues during discontinuous anaerobic digestion, Waste Management, 52, 295–301. https://doi.org/10.1016/j.wasman.2016.03.028
[16] Faostat, Crop Statistics, 2022. https://www.fao.org/faostat/en/#data/QC
[17] Fish W. W., Bruton B. D., Russo V. M. (2009). Watermelon juice: a promising feedstock supplement, diluent, and nitrogen supplement for ethanol biofuel production, Biotechnology for Biofuels, 2, 1-9. https://doi.org/10.1186/1754-6834-2-18
[18] Agger J. W., Nilsen P. J., Eijsink V. G. H., Horn S. J. (2014). On the determination of water content in biomass processing. Bioenergy Research, 7, 442–449. https://doi.org/10.1007/s12155-013-9388-2
[19] Dubois M., Gilles K., Hamilton J. K., Rebers P. A., Smith F. (1951). A colorimetric method for the determination of sugars. Nature, 168, 167. https://doi.org/10.1038/168167a0
[20] Bekele D. A., Geleta G. S. (2015). Iodometric determination of the ascorbic acid (Vitamin C) content of some fruits consumed in Jimma Town Community in Ethiopia. Research Journal of Chemical Sciences, 5, 60-63.
[21] Diakabana P., Kobawila S. C., Massengo V., Louembé D. (2013). Effet du degré de maturation sur la cinétique de fermentation éthylique de la pulpe de mangue cultivar Boko. Cameroon Journal of Experimental Biology, 9, 1-8. DOI: 10.4314/cajeb.v9i1.1.
[22] Massengo V., Loumouamou B. W., Diakabana P., Silou T. (2014). Ethanol production by fermentation of the pulp of the ‘‘Boko’’ mango. International Journal of Chemical Sciences and Technology, 4, 71-77.
[23] Sabba Gowda A., Njintang Y. N., Mbofung C. M. F. (2018). Study of production parameters of bioethanol from neem fruit pulp (Azadirachta indica). Global Journal of Engineering Science and Research Management, 59-70.
[24] Novidzro K. M., Agbodan K. A., Koumaglo K. H. (2013). Study of the performance of four strains of Saccharomyces cerevisiae on ethanol production with sucrose enriched musts. Journal de la Société Ouest Africaine de Chimie, 35, 1-7.
[25] Wehner T. C. (2008). Watermelon. In: Prohens J., Nuez F. Vegetables I. Handbook of Plant Breeding, vol 1. Springer, New York.
[26] Garcia-Gonzalez L., Geeraerd A. H., Spilimbergo S., Elst K., Van Ginneken L., Debevere J., Van Impe J. F., Devlieghere F. (2007). High pressure carbon dioxide inactivation of microorganisms in foods: the past, the present and the future. International Journal of Food and Microbiology. 10, 117, 1-28.
[27] Broach J. R. (2012). Nutritional control of growth and development in yeast, Genetics, 192, 73–105. https://doi.org/10.1534/genetics.111.135731
[28] Gobert A. (2019). Etude des besoins en azote des levures non Saccharomyces en vinification: impact sur les fermentations séquentielles. Thèse de doctorat Université Bourgogne Franche-Comte; 265 p.
[29] Booth, I. R. (2002). Stress and the single cell: Intrapopulation diversity is a mechanism to ensure survival upon exposure to stress. International Journal of Food Microbiology, 78, 19– 30. http://doi.org/10.1016/S0168-1605(02)00239-8
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    Marie-France, B., Batcha, O., Nyonuwosro, S. K., Abel, A. E., Hodo-Abalo, K. (2024). Valorization of Watermelon (Citrullus lanatus) into Bioethanol Using Several Yeast Strains of Saccharomyces Cerevisiae. American Journal of Applied Chemistry, 12(1), 15-21. https://doi.org/10.11648/j.ajac.20241201.12

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    ACS Style

    Marie-France, B.; Batcha, O.; Nyonuwosro, S. K.; Abel, A. E.; Hodo-Abalo, K. Valorization of Watermelon (Citrullus lanatus) into Bioethanol Using Several Yeast Strains of Saccharomyces Cerevisiae. Am. J. Appl. Chem. 2024, 12(1), 15-21. doi: 10.11648/j.ajac.20241201.12

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    AMA Style

    Marie-France B, Batcha O, Nyonuwosro SK, Abel AE, Hodo-Abalo K. Valorization of Watermelon (Citrullus lanatus) into Bioethanol Using Several Yeast Strains of Saccharomyces Cerevisiae. Am J Appl Chem. 2024;12(1):15-21. doi: 10.11648/j.ajac.20241201.12

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  • @article{10.11648/j.ajac.20241201.12,
      author = {Bakaï Marie-France and Ouadja Batcha and Segbeaya Kwamivi Nyonuwosro and Abli Essowèréou Abel and Kabissi Hodo-Abalo},
      title = {Valorization of Watermelon (Citrullus lanatus) into Bioethanol Using Several Yeast Strains of Saccharomyces Cerevisiae},
      journal = {American Journal of Applied Chemistry},
      volume = {12},
      number = {1},
      pages = {15-21},
      doi = {10.11648/j.ajac.20241201.12},
      url = {https://doi.org/10.11648/j.ajac.20241201.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20241201.12},
      abstract = {Watermelon is a fruit with very high losses, and its waste can attract pests. It’s therefore preferable to look for ways to recycle this waste. This study aims to valorize watermelon waste for the production of bioethanol by fermentation. The performance of six yeast strains of Saccharomyces Cerevisiae namely “Zymaflore X16, Actiflore F5, Zymaflore X5, Actiflore BO213, Zymaflore Delta, Zymaflore RX60 and Saf-Levure” were tested, in the presence and absence of urea as a growth factor. The results show that it is possible to obtain ethanol from watermelon waste. Among the six yeast strains studied, four see their fermentation performance improved in the presence of urea. The best yields are obtained for “X5 and RX60” strains with yields close to 18% (compared to less than 4,5% in the absence of urea for the same yeast strains). Nitrogen compounds are therefore nutrients that improve the fermenting power of yeast. The purity of ethanol obtained after distillation of fermentation must varies between 10 and 40% in the absence of urea and between 18 and 42% in the presence of urea. The watermelon from Togo therefore gives interesting and encouraging results for the production of bioethanol with yeast strains that stand out and whose use can be considered for the production of bioethanol on a larger scale. The results obtained will still require optimizing the conditions.
    },
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Valorization of Watermelon (Citrullus lanatus) into Bioethanol Using Several Yeast Strains of Saccharomyces Cerevisiae
    AU  - Bakaï Marie-France
    AU  - Ouadja Batcha
    AU  - Segbeaya Kwamivi Nyonuwosro
    AU  - Abli Essowèréou Abel
    AU  - Kabissi Hodo-Abalo
    Y1  - 2024/01/18
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    DO  - 10.11648/j.ajac.20241201.12
    T2  - American Journal of Applied Chemistry
    JF  - American Journal of Applied Chemistry
    JO  - American Journal of Applied Chemistry
    SP  - 15
    EP  - 21
    PB  - Science Publishing Group
    SN  - 2330-8745
    UR  - https://doi.org/10.11648/j.ajac.20241201.12
    AB  - Watermelon is a fruit with very high losses, and its waste can attract pests. It’s therefore preferable to look for ways to recycle this waste. This study aims to valorize watermelon waste for the production of bioethanol by fermentation. The performance of six yeast strains of Saccharomyces Cerevisiae namely “Zymaflore X16, Actiflore F5, Zymaflore X5, Actiflore BO213, Zymaflore Delta, Zymaflore RX60 and Saf-Levure” were tested, in the presence and absence of urea as a growth factor. The results show that it is possible to obtain ethanol from watermelon waste. Among the six yeast strains studied, four see their fermentation performance improved in the presence of urea. The best yields are obtained for “X5 and RX60” strains with yields close to 18% (compared to less than 4,5% in the absence of urea for the same yeast strains). Nitrogen compounds are therefore nutrients that improve the fermenting power of yeast. The purity of ethanol obtained after distillation of fermentation must varies between 10 and 40% in the absence of urea and between 18 and 42% in the presence of urea. The watermelon from Togo therefore gives interesting and encouraging results for the production of bioethanol with yeast strains that stand out and whose use can be considered for the production of bioethanol on a larger scale. The results obtained will still require optimizing the conditions.
    
    VL  - 12
    IS  - 1
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Author Information
  • Laboratory of Organic Chemistry and Environmental Sciences (LaCOSE), Department of Chemistry, Faculty of Science and Technology, Kara University, Kara, Togo

  • Laboratory of Biochemistry of Food and Nutrition (LABAN), Department of Life and earth Sciences, Faculty of Science and Technology, Kara University, Kara, Togo

  • Laboratory of Organic Chemistry and Environmental Sciences (LaCOSE), Department of Chemistry, Faculty of Science and Technology, Kara University, Kara, Togo

  • Laboratory of Biochemistry of Food and Nutrition (LABAN), Department of Life and earth Sciences, Faculty of Science and Technology, Kara University, Kara, Togo

  • Laboratory of Organic Chemistry and Environmental Sciences (LaCOSE), Department of Chemistry, Faculty of Science and Technology, Kara University, Kara, Togo

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