Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of <i>Culex pipiens</i> Complex Collected in Baljurashi Province, Saudi Arabia

Ehab Mohammed Abdella; Tariq Saeed Alghamdi; Khalid Mohammed Alghamdi; Jazem Abdullah Mahyoub

doi:doi:10.11648/j.ajz.20180101.12

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Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia

Ehab Mohammed Abdella, Tariq Saeed Alghamdi, Khalid Mohammed Alghamdi, Jazem Abdullah Mahyoub

Published in American Journal of Zoology (Volume 1, Issue 1)

Received: 7 May 2018 Accepted: 28 May 2018 Published: 14 June 2018

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Abstract

Some species of mosquitoes such as Culex pipiens complex are survive at different habitat conditions. They are considered of medically important vectors for some diseases, which causes a huge financial and medical problems to humans. From this point of view, species identification is the first step in the control of Culex pipiens complex. Previously identification of Culex pipiens complex is mainly done on the basis of a wide variety of morphological and biological characteristics. This can be difficult because diagnostic morphological features are often varied so little between species. So that in the present work, Culex species identification is performed by using DNA sequencing assay based on the mtDNA cytochrome oxidase-I (COX-I) gene. 56 mosquitoes specimens were collected from different infected areas in Baljurashi province, Al Baha, Saudi Arabia and grown for several generations in lab. COX-I gene amplification was carried out using the universal primers LCO1490 and HCO2198. Only 35 mosquito specimens had good amplified COXI target gene sequenced. These DNA sequences were compared with available sequences using basic alignment search tool in NCBI-Nucleotide database. Most of the examined specimens have high matching result in gene-bank with LC102132. However specimens T6, 8, 9, 15, 21, 45 & 51 have high similarity with JQ958371 Culex pipiens strain cf-3 and also specimen T35 has high similarity with KJ500032 Culex pipiens isolate 2AF. In conclusion, Sequence alignment and phylogenetic analysis of mitochondrial COX-I gene can be used as molecular tool for identification of Culex species.

Published in	American Journal of Zoology (Volume 1, Issue 1)
DOI	10.11648/j.ajz.20180101.12
Page(s)	7-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Culex pipiens Complex, Mitochondrial COX-I Gene, Sequence Alignment, Phylogenetic Analysis

References

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APA Style

Ehab Mohammed Abdella, Tariq Saeed Alghamdi, Khalid Mohammed Alghamdi, Jazem Abdullah Mahyoub. (2018). Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia. American Journal of Zoology, 1(1), 7-14. https://doi.org/10.11648/j.ajz.20180101.12

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ACS Style

Ehab Mohammed Abdella; Tariq Saeed Alghamdi; Khalid Mohammed Alghamdi; Jazem Abdullah Mahyoub. Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia. Am. J. Zool. 2018, 1(1), 7-14. doi: 10.11648/j.ajz.20180101.12

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AMA Style

Ehab Mohammed Abdella, Tariq Saeed Alghamdi, Khalid Mohammed Alghamdi, Jazem Abdullah Mahyoub. Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia. Am J Zool. 2018;1(1):7-14. doi: 10.11648/j.ajz.20180101.12

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@article{10.11648/j.ajz.20180101.12,
  author = {Ehab Mohammed Abdella and Tariq Saeed Alghamdi and Khalid Mohammed Alghamdi and Jazem Abdullah Mahyoub},
  title = {Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia},
  journal = {American Journal of Zoology},
  volume = {1},
  number = {1},
  pages = {7-14},
  doi = {10.11648/j.ajz.20180101.12},
  url = {https://doi.org/10.11648/j.ajz.20180101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajz.20180101.12},
  abstract = {Some species of mosquitoes such as Culex pipiens complex are survive at different habitat conditions. They are considered of medically important vectors for some diseases, which causes a huge financial and medical problems to humans. From this point of view, species identification is the first step in the control of Culex pipiens complex. Previously identification of Culex pipiens complex is mainly done on the basis of a wide variety of morphological and biological characteristics. This can be difficult because diagnostic morphological features are often varied so little between species. So that in the present work, Culex species identification is performed by using DNA sequencing assay based on the mtDNA cytochrome oxidase-I (COX-I) gene. 56 mosquitoes specimens were collected from different infected areas in Baljurashi province, Al Baha, Saudi Arabia and grown for several generations in lab. COX-I gene amplification was carried out using the universal primers LCO1490 and HCO2198. Only 35 mosquito specimens had good amplified COXI target gene sequenced. These DNA sequences were compared with available sequences using basic alignment search tool in NCBI-Nucleotide database. Most of the examined specimens have high matching result in gene-bank with LC102132. However specimens T6, 8, 9, 15, 21, 45 & 51 have high similarity with JQ958371 Culex pipiens strain cf-3 and also specimen T35 has high similarity with KJ500032 Culex pipiens isolate 2AF. In conclusion, Sequence alignment and phylogenetic analysis of mitochondrial COX-I gene can be used as molecular tool for identification of Culex species.},
 year = {2018}
}

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TY - JOUR
T1 - Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia
AU - Ehab Mohammed Abdella
AU - Tariq Saeed Alghamdi
AU - Khalid Mohammed Alghamdi
AU - Jazem Abdullah Mahyoub
Y1 - 2018/06/14
PY - 2018
N1 - https://doi.org/10.11648/j.ajz.20180101.12
DO - 10.11648/j.ajz.20180101.12
T2 - American Journal of Zoology
JF - American Journal of Zoology
JO - American Journal of Zoology
SP - 7
EP - 14
PB - Science Publishing Group
SN - 2994-7413
UR - https://doi.org/10.11648/j.ajz.20180101.12
AB - Some species of mosquitoes such as Culex pipiens complex are survive at different habitat conditions. They are considered of medically important vectors for some diseases, which causes a huge financial and medical problems to humans. From this point of view, species identification is the first step in the control of Culex pipiens complex. Previously identification of Culex pipiens complex is mainly done on the basis of a wide variety of morphological and biological characteristics. This can be difficult because diagnostic morphological features are often varied so little between species. So that in the present work, Culex species identification is performed by using DNA sequencing assay based on the mtDNA cytochrome oxidase-I (COX-I) gene. 56 mosquitoes specimens were collected from different infected areas in Baljurashi province, Al Baha, Saudi Arabia and grown for several generations in lab. COX-I gene amplification was carried out using the universal primers LCO1490 and HCO2198. Only 35 mosquito specimens had good amplified COXI target gene sequenced. These DNA sequences were compared with available sequences using basic alignment search tool in NCBI-Nucleotide database. Most of the examined specimens have high matching result in gene-bank with LC102132. However specimens T6, 8, 9, 15, 21, 45 & 51 have high similarity with JQ958371 Culex pipiens strain cf-3 and also specimen T35 has high similarity with KJ500032 Culex pipiens isolate 2AF. In conclusion, Sequence alignment and phylogenetic analysis of mitochondrial COX-I gene can be used as molecular tool for identification of Culex species.
VL - 1
IS - 1
ER -

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Author Information

Ehab Mohammed Abdella

Biology Department, Faculty of Science, Al Baha University, Al Baha, Saudi Arabia
Tariq Saeed Alghamdi

Biology Department, Faculty of Science, Al Baha University, Al Baha, Saudi Arabia
Khalid Mohammed Alghamdi

Biology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
Jazem Abdullah Mahyoub

Biology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

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Ehab Mohammed Abdella, Tariq Saeed Alghamdi, Khalid Mohammed Alghamdi, Jazem Abdullah Mahyoub. (2018). Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia. American Journal of Zoology, 1(1), 7-14. https://doi.org/10.11648/j.ajz.20180101.12

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Ehab Mohammed Abdella; Tariq Saeed Alghamdi; Khalid Mohammed Alghamdi; Jazem Abdullah Mahyoub. Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia. Am. J. Zool. 2018, 1(1), 7-14. doi: 10.11648/j.ajz.20180101.12

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AMA Style

Ehab Mohammed Abdella, Tariq Saeed Alghamdi, Khalid Mohammed Alghamdi, Jazem Abdullah Mahyoub. Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia. Am J Zool. 2018;1(1):7-14. doi: 10.11648/j.ajz.20180101.12

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@article{10.11648/j.ajz.20180101.12,
  author = {Ehab Mohammed Abdella and Tariq Saeed Alghamdi and Khalid Mohammed Alghamdi and Jazem Abdullah Mahyoub},
  title = {Cytochrome Oxidase-I Gene Sequencing Approaches to Identification of Culex pipiens Complex Collected in Baljurashi Province, Saudi Arabia},
  journal = {American Journal of Zoology},
  volume = {1},
  number = {1},
  pages = {7-14},
  doi = {10.11648/j.ajz.20180101.12},
  url = {https://doi.org/10.11648/j.ajz.20180101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajz.20180101.12},
  abstract = {Some species of mosquitoes such as Culex pipiens complex are survive at different habitat conditions. They are considered of medically important vectors for some diseases, which causes a huge financial and medical problems to humans. From this point of view, species identification is the first step in the control of Culex pipiens complex. Previously identification of Culex pipiens complex is mainly done on the basis of a wide variety of morphological and biological characteristics. This can be difficult because diagnostic morphological features are often varied so little between species. So that in the present work, Culex species identification is performed by using DNA sequencing assay based on the mtDNA cytochrome oxidase-I (COX-I) gene. 56 mosquitoes specimens were collected from different infected areas in Baljurashi province, Al Baha, Saudi Arabia and grown for several generations in lab. COX-I gene amplification was carried out using the universal primers LCO1490 and HCO2198. Only 35 mosquito specimens had good amplified COXI target gene sequenced. These DNA sequences were compared with available sequences using basic alignment search tool in NCBI-Nucleotide database. Most of the examined specimens have high matching result in gene-bank with LC102132. However specimens T6, 8, 9, 15, 21, 45 & 51 have high similarity with JQ958371 Culex pipiens strain cf-3 and also specimen T35 has high similarity with KJ500032 Culex pipiens isolate 2AF. In conclusion, Sequence alignment and phylogenetic analysis of mitochondrial COX-I gene can be used as molecular tool for identification of Culex species.},
 year = {2018}
}

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