@article{10.11648/j.ajz.20220502.11,
  author = {Gelana Keno and Belay Habtegebriel and Ferdu Azerefegne},
  title = {Evaluation of Ethiopian Entomopathogenic Fungi Isolates Against the Two-Spotted Spider Mite, Tetranychus urticae Koch on Tomato, Solanum lycopersicum L.},
  journal = {American Journal of Zoology},
  volume = {5},
  number = {2},
  pages = {11-19},
  doi = {10.11648/j.ajz.20220502.11},
  url = {https://doi.org/10.11648/j.ajz.20220502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajz.20220502.11},
  abstract = {Two spotted spidermite (TSSM), Tetranychus urticae (Koch, 1836) is economically important in greenhouse grown tomato in Ethiopia. This study entomopathogenic fungi (EPF) such as Metarhizium anisopliae (Metschnikoff, 1883) and Beauveria bassiana (Balsamo, 1912) isolates evaluated for the management of the two spotted spider mites under both laboratory and glasshouse controlled environmental conditions. Six fungal newly isolated from the soil and one existing isolate were evaluated and identified in the laboratory for virulence against T. urticae. Spore dilutions were prepared in 0.01% Tween 80. Adults and eggs were sprayed with 1 ml of conidial suspension adjusted to 1×108 conidia/ml. The mites those treated were kept under controlled conditions at 25°C and 60–70% RH and the number of dead mites was recorded daily on tomato leaf. All the isolates tested were infected and killed adults and eggs of spider mites at a concentration of 1×108 conidia ml-1 after eight and seven days respectively, but had varying virulence on adults ranged from 33% to 93% and on egg ranged from 32% to 87%. Two B. bassiana isolates, GF3 and 9604, which resulted in 93% and 85% mortality of adults 8 days post inoculation, respectively were categorized as highly virulent whereas M. anisopliae MF3 and AF5 which resulted in 64% and 54% mortality were considered as moderately virulent isolates. Similarly, B. bassiana AF2, BF4 and KF3 which resulted in mortality of 47%, 36% and 33%, respectively, were considered as weak isolates against the two spotted spider mites. The highly virulent isolates were further evaluated under glasshouse condition on potted tomato plants for their virulence against T. urticae. Isolate GF3 caused the highest mortality (78%) followed by 9604 which caused 70% mortality after seven days of inoculation. From this study the native M. anisopliae and B. bassiana isolates have potential to kill the TSSM both eggs and adults’ stages of the pest. Thus why, EPF isolates can be used as an alternative to chemical treatments.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Evaluation of Ethiopian Entomopathogenic Fungi Isolates Against the Two-Spotted Spider Mite, Tetranychus urticae Koch on Tomato, Solanum lycopersicum L.
AU  - Gelana Keno
AU  - Belay Habtegebriel
AU  - Ferdu Azerefegne
Y1  - 2022/04/28
PY  - 2022
N1  - https://doi.org/10.11648/j.ajz.20220502.11
DO  - 10.11648/j.ajz.20220502.11
T2  - American Journal of Zoology
JF  - American Journal of Zoology
JO  - American Journal of Zoology
SP  - 11
EP  - 19
PB  - Science Publishing Group
SN  - 2994-7413
UR  - https://doi.org/10.11648/j.ajz.20220502.11
AB  - Two spotted spidermite (TSSM), Tetranychus urticae (Koch, 1836) is economically important in greenhouse grown tomato in Ethiopia. This study entomopathogenic fungi (EPF) such as Metarhizium anisopliae (Metschnikoff, 1883) and Beauveria bassiana (Balsamo, 1912) isolates evaluated for the management of the two spotted spider mites under both laboratory and glasshouse controlled environmental conditions. Six fungal newly isolated from the soil and one existing isolate were evaluated and identified in the laboratory for virulence against T. urticae. Spore dilutions were prepared in 0.01% Tween 80. Adults and eggs were sprayed with 1 ml of conidial suspension adjusted to 1×108 conidia/ml. The mites those treated were kept under controlled conditions at 25°C and 60–70% RH and the number of dead mites was recorded daily on tomato leaf. All the isolates tested were infected and killed adults and eggs of spider mites at a concentration of 1×108 conidia ml-1 after eight and seven days respectively, but had varying virulence on adults ranged from 33% to 93% and on egg ranged from 32% to 87%. Two B. bassiana isolates, GF3 and 9604, which resulted in 93% and 85% mortality of adults 8 days post inoculation, respectively were categorized as highly virulent whereas M. anisopliae MF3 and AF5 which resulted in 64% and 54% mortality were considered as moderately virulent isolates. Similarly, B. bassiana AF2, BF4 and KF3 which resulted in mortality of 47%, 36% and 33%, respectively, were considered as weak isolates against the two spotted spider mites. The highly virulent isolates were further evaluated under glasshouse condition on potted tomato plants for their virulence against T. urticae. Isolate GF3 caused the highest mortality (78%) followed by 9604 which caused 70% mortality after seven days of inoculation. From this study the native M. anisopliae and B. bassiana isolates have potential to kill the TSSM both eggs and adults’ stages of the pest. Thus why, EPF isolates can be used as an alternative to chemical treatments.
VL  - 5
IS  - 2
ER  - 

Copy | Download