A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis

Zexian Chen; Yongle Chen; Xiaowen He; Ping Lan

doi:doi:10.11648/j.ijcems.20180404.12

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A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis

Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 4, Issue 4)

Received: 6 September 2018 Accepted: 20 September 2018 Published: 12 October 2018

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Abstract

Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 4, Issue 4)
DOI	10.11648/j.ijcems.20180404.12
Page(s)	63-67
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Inflammatory Bowel Disease, Tumorigenesis, Mice Model

References

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APA Style

Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan. (2018). A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. International Journal of Clinical and Experimental Medical Sciences, 4(4), 63-67. https://doi.org/10.11648/j.ijcems.20180404.12

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ACS Style

Zexian Chen; Yongle Chen; Xiaowen He; Ping Lan. A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. Int. J. Clin. Exp. Med. Sci. 2018, 4(4), 63-67. doi: 10.11648/j.ijcems.20180404.12

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AMA Style

Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan. A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. Int J Clin Exp Med Sci. 2018;4(4):63-67. doi: 10.11648/j.ijcems.20180404.12

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@article{10.11648/j.ijcems.20180404.12,
  author = {Zexian Chen and Yongle Chen and Xiaowen He and Ping Lan},
  title = {A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {4},
  number = {4},
  pages = {63-67},
  doi = {10.11648/j.ijcems.20180404.12},
  url = {https://doi.org/10.11648/j.ijcems.20180404.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20180404.12},
  abstract = {Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.},
 year = {2018}
}

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TY  - JOUR
T1  - A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis
AU  - Zexian Chen
AU  - Yongle Chen
AU  - Xiaowen He
AU  - Ping Lan
Y1  - 2018/10/12
PY  - 2018
N1  - https://doi.org/10.11648/j.ijcems.20180404.12
DO  - 10.11648/j.ijcems.20180404.12
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 63
EP  - 67
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20180404.12
AB  - Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.
VL  - 4
IS  - 4
ER  -

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Author Information

Zexian Chen

Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Yongle Chen

Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Xiaowen He

Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Ping Lan

Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

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Plain Text BibTeX RIS

APA Style

Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan. (2018). A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. International Journal of Clinical and Experimental Medical Sciences, 4(4), 63-67. https://doi.org/10.11648/j.ijcems.20180404.12

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ACS Style

Zexian Chen; Yongle Chen; Xiaowen He; Ping Lan. A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. Int. J. Clin. Exp. Med. Sci. 2018, 4(4), 63-67. doi: 10.11648/j.ijcems.20180404.12

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AMA Style

Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan. A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. Int J Clin Exp Med Sci. 2018;4(4):63-67. doi: 10.11648/j.ijcems.20180404.12

Copy | Download

@article{10.11648/j.ijcems.20180404.12,
  author = {Zexian Chen and Yongle Chen and Xiaowen He and Ping Lan},
  title = {A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {4},
  number = {4},
  pages = {63-67},
  doi = {10.11648/j.ijcems.20180404.12},
  url = {https://doi.org/10.11648/j.ijcems.20180404.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20180404.12},
  abstract = {Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis
AU  - Zexian Chen
AU  - Yongle Chen
AU  - Xiaowen He
AU  - Ping Lan
Y1  - 2018/10/12
PY  - 2018
N1  - https://doi.org/10.11648/j.ijcems.20180404.12
DO  - 10.11648/j.ijcems.20180404.12
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 63
EP  - 67
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20180404.12
AB  - Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.
VL  - 4
IS  - 4
ER  -

Copy | Download