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Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm

Received: 22 May 2019     Accepted: 28 June 2019     Published: 24 July 2019
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Abstract

The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light.

Published in International Journal of Microbiology and Biotechnology (Volume 4, Issue 3)
DOI 10.11648/j.ijmb.20190403.11
Page(s) 55-63
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Enterococcus faecalis, Escherichia coli, Light Intensity, Mercury Chloride, Biodegradable Organic Compound, Aquatic Microcosm

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Cite This Article
  • APA Style

    Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, et al. (2019). Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. International Journal of Microbiology and Biotechnology, 4(3), 55-63. https://doi.org/10.11648/j.ijmb.20190403.11

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    Pierrette Ngo Bahebeck; Claire Stephane Metsopkeng; Joelle Signe MBiada; Chrétien Lontsi Djimeli; Antoine Tamsa Arfao, et al. Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. Int. J. Microbiol. Biotechnol. 2019, 4(3), 55-63. doi: 10.11648/j.ijmb.20190403.11

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    AMA Style

    Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, et al. Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. Int J Microbiol Biotechnol. 2019;4(3):55-63. doi: 10.11648/j.ijmb.20190403.11

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  • @article{10.11648/j.ijmb.20190403.11,
      author = {Pierrette Ngo Bahebeck and Claire Stephane Metsopkeng and Joelle Signe MBiada and Chrétien Lontsi Djimeli and Antoine Tamsa Arfao and Paul Alain Nana and Olive Vivien Noah Ewoti and Luciane Marlyse Moungang and Genevieve Bricheux and Télesphore Sime-Ngando and Moïse Nola},
      title = {Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {4},
      number = {3},
      pages = {55-63},
      doi = {10.11648/j.ijmb.20190403.11},
      url = {https://doi.org/10.11648/j.ijmb.20190403.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190403.11},
      abstract = {The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm
    AU  - Pierrette Ngo Bahebeck
    AU  - Claire Stephane Metsopkeng
    AU  - Joelle Signe MBiada
    AU  - Chrétien Lontsi Djimeli
    AU  - Antoine Tamsa Arfao
    AU  - Paul Alain Nana
    AU  - Olive Vivien Noah Ewoti
    AU  - Luciane Marlyse Moungang
    AU  - Genevieve Bricheux
    AU  - Télesphore Sime-Ngando
    AU  - Moïse Nola
    Y1  - 2019/07/24
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijmb.20190403.11
    DO  - 10.11648/j.ijmb.20190403.11
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 55
    EP  - 63
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20190403.11
    AB  - The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

  • Laboratory 'Microorganisms: Genome and Environment', Clermont Auvergne University, Aubière Cedex, France

  • Laboratory 'Microorganisms: Genome and Environment', Clermont Auvergne University, Aubière Cedex, France

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