Antibiotic Resistance Pattern of <i>Escherichia coli</i> Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria

Nasir Tukur Dabo; Bashir Muhammad; Habeeb Kayode Saka; Zaharaddin Muhammad Kalgo; Rasaki Adewole Raheem

doi:doi:10.11648/j.ijmb.20190403.15

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Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria

Nasir Tukur Dabo, Bashir Muhammad, Habeeb Kayode Saka, Zaharaddin Muhammad Kalgo, Rasaki Adewole Raheem

Published in International Journal of Microbiology and Biotechnology (Volume 4, Issue 3)

Received: 21 May 2019 Accepted: 25 June 2019 Published: 10 September 2019

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Abstract

Antibiotic resistance in bacterial pathogens is a global health challenge linked to high morbidity and mortality. This study was carried out among under-five children attending three major hospitals in Kano State namely: Murtala Muhammad Specialists Hospital (MMSH), Wudil General Hospital (WGH) and Bichi General Hospital (BGH), representing the three senatorial districts. Rectal swab specimens from 400 diarrhoeic and 50 non-diarrhoeic children were collected with a sterile transport swab containing Carry-Blair Medium. These were inoculated onto MacConckey and Salmonella-Shigella Agar and incubated at 37°C for 18-24 hours for isolation of bacteria. Bacterial isolates were subjected to battery of biochemical tests (IMViC and KIA) for the identification of Escherichia coli. Antimicrobial susceptibility test (AST) was carried out on E. coli isolated using modified Kirby-Bauer method. The findings revealed 74% and 66% E. coli recovery from case and control subjects respectively. The AST revealed trimethoprim-sulfamethoxazole 75.1% was the most resisted antibiotic, significantly different between the case subjects (P-value=0.031), 83% were resistant to at least one class of antibiotic, 44.4% resistant to two classes of antibiotics and significantly higher in the control group (P-value=0.006) and 8.0% MDR rate. Female subjects shows higher resistance to the tested antibiotics but the differences were only significant in amoxicillin-clavulanic acid (49.6% versus 32.9%; P-value=0.003) and trimethoprim-sulfamethoxazole (91.7% versus 57.1%; P-value=0.037) in the case and control group respectively. It can be concluded that E. coli is the predominant agent associated with diarrhoea in Kano children and high resistance of trimethoprim-sulfamethoxazole is noteworthy. Hence the need to revise the current diarrhoea treatment regimen for Kano children.

Published in	International Journal of Microbiology and Biotechnology (Volume 4, Issue 3)
DOI	10.11648/j.ijmb.20190403.15
Page(s)	94-102
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Escherichia coli, Antibiogram, Diarrhoeic, Resistance, Kano

References

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Nasir Tukur Dabo, Bashir Muhammad, Habeeb Kayode Saka, Zaharaddin Muhammad Kalgo, Rasaki Adewole Raheem. (2019). Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria. International Journal of Microbiology and Biotechnology, 4(3), 94-102. https://doi.org/10.11648/j.ijmb.20190403.15

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Nasir Tukur Dabo; Bashir Muhammad; Habeeb Kayode Saka; Zaharaddin Muhammad Kalgo; Rasaki Adewole Raheem. Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria. Int. J. Microbiol. Biotechnol. 2019, 4(3), 94-102. doi: 10.11648/j.ijmb.20190403.15

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Nasir Tukur Dabo, Bashir Muhammad, Habeeb Kayode Saka, Zaharaddin Muhammad Kalgo, Rasaki Adewole Raheem. Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria. Int J Microbiol Biotechnol. 2019;4(3):94-102. doi: 10.11648/j.ijmb.20190403.15

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@article{10.11648/j.ijmb.20190403.15,
  author = {Nasir Tukur Dabo and Bashir Muhammad and Habeeb Kayode Saka and Zaharaddin Muhammad Kalgo and Rasaki Adewole Raheem},
  title = {Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {4},
  number = {3},
  pages = {94-102},
  doi = {10.11648/j.ijmb.20190403.15},
  url = {https://doi.org/10.11648/j.ijmb.20190403.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190403.15},
  abstract = {Antibiotic resistance in bacterial pathogens is a global health challenge linked to high morbidity and mortality. This study was carried out among under-five children attending three major hospitals in Kano State namely: Murtala Muhammad Specialists Hospital (MMSH), Wudil General Hospital (WGH) and Bichi General Hospital (BGH), representing the three senatorial districts. Rectal swab specimens from 400 diarrhoeic and 50 non-diarrhoeic children were collected with a sterile transport swab containing Carry-Blair Medium. These were inoculated onto MacConckey and Salmonella-Shigella Agar and incubated at 37°C for 18-24 hours for isolation of bacteria. Bacterial isolates were subjected to battery of biochemical tests (IMViC and KIA) for the identification of Escherichia coli. Antimicrobial susceptibility test (AST) was carried out on E. coli isolated using modified Kirby-Bauer method. The findings revealed 74% and 66% E. coli recovery from case and control subjects respectively. The AST revealed trimethoprim-sulfamethoxazole 75.1% was the most resisted antibiotic, significantly different between the case subjects (P-value=0.031), 83% were resistant to at least one class of antibiotic, 44.4% resistant to two classes of antibiotics and significantly higher in the control group (P-value=0.006) and 8.0% MDR rate. Female subjects shows higher resistance to the tested antibiotics but the differences were only significant in amoxicillin-clavulanic acid (49.6% versus 32.9%; P-value=0.003) and trimethoprim-sulfamethoxazole (91.7% versus 57.1%; P-value=0.037) in the case and control group respectively. It can be concluded that E. coli is the predominant agent associated with diarrhoea in Kano children and high resistance of trimethoprim-sulfamethoxazole is noteworthy. Hence the need to revise the current diarrhoea treatment regimen for Kano children.},
 year = {2019}
}

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TY - JOUR
T1 - Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria
AU - Nasir Tukur Dabo
AU - Bashir Muhammad
AU - Habeeb Kayode Saka
AU - Zaharaddin Muhammad Kalgo
AU - Rasaki Adewole Raheem
Y1 - 2019/09/10
PY - 2019
N1 - https://doi.org/10.11648/j.ijmb.20190403.15
DO - 10.11648/j.ijmb.20190403.15
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 94
EP - 102
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20190403.15
AB - Antibiotic resistance in bacterial pathogens is a global health challenge linked to high morbidity and mortality. This study was carried out among under-five children attending three major hospitals in Kano State namely: Murtala Muhammad Specialists Hospital (MMSH), Wudil General Hospital (WGH) and Bichi General Hospital (BGH), representing the three senatorial districts. Rectal swab specimens from 400 diarrhoeic and 50 non-diarrhoeic children were collected with a sterile transport swab containing Carry-Blair Medium. These were inoculated onto MacConckey and Salmonella-Shigella Agar and incubated at 37°C for 18-24 hours for isolation of bacteria. Bacterial isolates were subjected to battery of biochemical tests (IMViC and KIA) for the identification of Escherichia coli. Antimicrobial susceptibility test (AST) was carried out on E. coli isolated using modified Kirby-Bauer method. The findings revealed 74% and 66% E. coli recovery from case and control subjects respectively. The AST revealed trimethoprim-sulfamethoxazole 75.1% was the most resisted antibiotic, significantly different between the case subjects (P-value=0.031), 83% were resistant to at least one class of antibiotic, 44.4% resistant to two classes of antibiotics and significantly higher in the control group (P-value=0.006) and 8.0% MDR rate. Female subjects shows higher resistance to the tested antibiotics but the differences were only significant in amoxicillin-clavulanic acid (49.6% versus 32.9%; P-value=0.003) and trimethoprim-sulfamethoxazole (91.7% versus 57.1%; P-value=0.037) in the case and control group respectively. It can be concluded that E. coli is the predominant agent associated with diarrhoea in Kano children and high resistance of trimethoprim-sulfamethoxazole is noteworthy. Hence the need to revise the current diarrhoea treatment regimen for Kano children.
VL - 4
IS - 3
ER -

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Author Information

Nasir Tukur Dabo

Department of Biological Sciences, Bayero University Kano, Kano, Nigeria
Bashir Muhammad

Department of Microbiology, Bayero University Kano, Kano, Nigeria
Habeeb Kayode Saka

Department of Microbiology, Bayero University Kano, Kano, Nigeria
Zaharaddin Muhammad Kalgo

Department of Microbiology, Federal University Birnin Kebbi, Kebbi State, Nigeria
Rasaki Adewole Raheem

Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Nigeria

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Nasir Tukur Dabo, Bashir Muhammad, Habeeb Kayode Saka, Zaharaddin Muhammad Kalgo, Rasaki Adewole Raheem. (2019). Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria. International Journal of Microbiology and Biotechnology, 4(3), 94-102. https://doi.org/10.11648/j.ijmb.20190403.15

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Nasir Tukur Dabo; Bashir Muhammad; Habeeb Kayode Saka; Zaharaddin Muhammad Kalgo; Rasaki Adewole Raheem. Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria. Int. J. Microbiol. Biotechnol. 2019, 4(3), 94-102. doi: 10.11648/j.ijmb.20190403.15

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Nasir Tukur Dabo, Bashir Muhammad, Habeeb Kayode Saka, Zaharaddin Muhammad Kalgo, Rasaki Adewole Raheem. Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria. Int J Microbiol Biotechnol. 2019;4(3):94-102. doi: 10.11648/j.ijmb.20190403.15

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@article{10.11648/j.ijmb.20190403.15,
  author = {Nasir Tukur Dabo and Bashir Muhammad and Habeeb Kayode Saka and Zaharaddin Muhammad Kalgo and Rasaki Adewole Raheem},
  title = {Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {4},
  number = {3},
  pages = {94-102},
  doi = {10.11648/j.ijmb.20190403.15},
  url = {https://doi.org/10.11648/j.ijmb.20190403.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190403.15},
  abstract = {Antibiotic resistance in bacterial pathogens is a global health challenge linked to high morbidity and mortality. This study was carried out among under-five children attending three major hospitals in Kano State namely: Murtala Muhammad Specialists Hospital (MMSH), Wudil General Hospital (WGH) and Bichi General Hospital (BGH), representing the three senatorial districts. Rectal swab specimens from 400 diarrhoeic and 50 non-diarrhoeic children were collected with a sterile transport swab containing Carry-Blair Medium. These were inoculated onto MacConckey and Salmonella-Shigella Agar and incubated at 37°C for 18-24 hours for isolation of bacteria. Bacterial isolates were subjected to battery of biochemical tests (IMViC and KIA) for the identification of Escherichia coli. Antimicrobial susceptibility test (AST) was carried out on E. coli isolated using modified Kirby-Bauer method. The findings revealed 74% and 66% E. coli recovery from case and control subjects respectively. The AST revealed trimethoprim-sulfamethoxazole 75.1% was the most resisted antibiotic, significantly different between the case subjects (P-value=0.031), 83% were resistant to at least one class of antibiotic, 44.4% resistant to two classes of antibiotics and significantly higher in the control group (P-value=0.006) and 8.0% MDR rate. Female subjects shows higher resistance to the tested antibiotics but the differences were only significant in amoxicillin-clavulanic acid (49.6% versus 32.9%; P-value=0.003) and trimethoprim-sulfamethoxazole (91.7% versus 57.1%; P-value=0.037) in the case and control group respectively. It can be concluded that E. coli is the predominant agent associated with diarrhoea in Kano children and high resistance of trimethoprim-sulfamethoxazole is noteworthy. Hence the need to revise the current diarrhoea treatment regimen for Kano children.},
 year = {2019}
}

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