Extended spectrum beta-lactamase prevalence is still on the increase across the world and has been implicated in urinary tract infections in Nigeria. TEM, SHV and CTX-M are becoming more common with CTX-M-15 becoming more significant as it is associated with complicated urinary tract infections. This study was conducted to evaluate distribution of blaTEM, blaCTX-M-15 and blaSHV genes among Enterobacter cloacae, E. coli and Klebsiella pneumoniae. A total of 660 urine samples were collected from pregnant women in 3 general hospitals from Akwa Ibom state. Enterobacter cloacae, E. coli and Klebsiella pneumoniae were identified using Microbact 24E. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility. The presence of ESBL was detected using Double Disk Synergy Test (DDST) and CHROMagar ESBL, respectively. Plasmid extraction was carried out following the protocol of ZR Plasmid Miniprep-Classic extraction kit. The blaCTX-M-15, blaTEM and blaSHV was identified by PCR with specific primers in selected 50 ESBL producing isolates. A total of 252 clinical isolates were collected from three General Hospitals in South-South, Nigeria. ESBLs were found in 231 (92%) isolates. blaCTX-M-15 was the commonest genotype (84%), followed by blaSHV (60%) and blaTEM (60%). ESBL positive strains of E. cloacae, E. coli and K pneumoniae are increasingly found in isolates from pregnant women. The current study demonstrated the predominance of gene encoding blaCTX-M-15 with a percentage frequency of (84%) commonly with plasmid in the setting.
| Published in | International Journal of Microbiology and Biotechnology (Volume 5, Issue 2) |
| DOI | 10.11648/j.ijmb.20200502.12 |
| Page(s) | 48-54 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
ESBL blagene, PCR, UTI
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APA Style
Florence Zion Uyanga, Emmanuel Olufemi Ekundayo, Emmanuel Onwubiko Nwankwo, Inimfon Akaninyene Ibanga. (2020). Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria. International Journal of Microbiology and Biotechnology, 5(2), 48-54. https://doi.org/10.11648/j.ijmb.20200502.12
ACS Style
Florence Zion Uyanga; Emmanuel Olufemi Ekundayo; Emmanuel Onwubiko Nwankwo; Inimfon Akaninyene Ibanga. Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria. Int. J. Microbiol. Biotechnol. 2020, 5(2), 48-54. doi: 10.11648/j.ijmb.20200502.12
AMA Style
Florence Zion Uyanga, Emmanuel Olufemi Ekundayo, Emmanuel Onwubiko Nwankwo, Inimfon Akaninyene Ibanga. Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria. Int J Microbiol Biotechnol. 2020;5(2):48-54. doi: 10.11648/j.ijmb.20200502.12
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Download@article{10.11648/j.ijmb.20200502.12,
author = {Florence Zion Uyanga and Emmanuel Olufemi Ekundayo and Emmanuel Onwubiko Nwankwo and Inimfon Akaninyene Ibanga},
title = {Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria},
journal = {International Journal of Microbiology and Biotechnology},
volume = {5},
number = {2},
pages = {48-54},
doi = {10.11648/j.ijmb.20200502.12},
url = {https://doi.org/10.11648/j.ijmb.20200502.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200502.12},
abstract = {Extended spectrum beta-lactamase prevalence is still on the increase across the world and has been implicated in urinary tract infections in Nigeria. TEM, SHV and CTX-M are becoming more common with CTX-M-15 becoming more significant as it is associated with complicated urinary tract infections. This study was conducted to evaluate distribution of blaTEM, blaCTX-M-15 and blaSHV genes among Enterobacter cloacae, E. coli and Klebsiella pneumoniae. A total of 660 urine samples were collected from pregnant women in 3 general hospitals from Akwa Ibom state. Enterobacter cloacae, E. coli and Klebsiella pneumoniae were identified using Microbact 24E. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility. The presence of ESBL was detected using Double Disk Synergy Test (DDST) and CHROMagar ESBL, respectively. Plasmid extraction was carried out following the protocol of ZR Plasmid Miniprep-Classic extraction kit. The blaCTX-M-15, blaTEM and blaSHV was identified by PCR with specific primers in selected 50 ESBL producing isolates. A total of 252 clinical isolates were collected from three General Hospitals in South-South, Nigeria. ESBLs were found in 231 (92%) isolates. blaCTX-M-15 was the commonest genotype (84%), followed by blaSHV (60%) and blaTEM (60%). ESBL positive strains of E. cloacae, E. coli and K pneumoniae are increasingly found in isolates from pregnant women. The current study demonstrated the predominance of gene encoding blaCTX-M-15 with a percentage frequency of (84%) commonly with plasmid in the setting.},
year = {2020}
}
TY - JOUR T1 - Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria AU - Florence Zion Uyanga AU - Emmanuel Olufemi Ekundayo AU - Emmanuel Onwubiko Nwankwo AU - Inimfon Akaninyene Ibanga Y1 - 2020/04/21 PY - 2020 N1 - https://doi.org/10.11648/j.ijmb.20200502.12 DO - 10.11648/j.ijmb.20200502.12 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 48 EP - 54 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20200502.12 AB - Extended spectrum beta-lactamase prevalence is still on the increase across the world and has been implicated in urinary tract infections in Nigeria. TEM, SHV and CTX-M are becoming more common with CTX-M-15 becoming more significant as it is associated with complicated urinary tract infections. This study was conducted to evaluate distribution of blaTEM, blaCTX-M-15 and blaSHV genes among Enterobacter cloacae, E. coli and Klebsiella pneumoniae. A total of 660 urine samples were collected from pregnant women in 3 general hospitals from Akwa Ibom state. Enterobacter cloacae, E. coli and Klebsiella pneumoniae were identified using Microbact 24E. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility. The presence of ESBL was detected using Double Disk Synergy Test (DDST) and CHROMagar ESBL, respectively. Plasmid extraction was carried out following the protocol of ZR Plasmid Miniprep-Classic extraction kit. The blaCTX-M-15, blaTEM and blaSHV was identified by PCR with specific primers in selected 50 ESBL producing isolates. A total of 252 clinical isolates were collected from three General Hospitals in South-South, Nigeria. ESBLs were found in 231 (92%) isolates. blaCTX-M-15 was the commonest genotype (84%), followed by blaSHV (60%) and blaTEM (60%). ESBL positive strains of E. cloacae, E. coli and K pneumoniae are increasingly found in isolates from pregnant women. The current study demonstrated the predominance of gene encoding blaCTX-M-15 with a percentage frequency of (84%) commonly with plasmid in the setting. VL - 5 IS - 2 ER -
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