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Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates

Received: 31 October 2022     Accepted: 21 November 2022     Published: 8 December 2022
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Abstract

To determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of chitosan from cockroach and cricket against bacterial isolates, Staphylococccus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Streptococcus pyogenes, Bacillus subtilis and Pseudomonas aeruginosa. The antimicrobial efficacy of chitosan was determined by the standard method of Clinical and Laboratory Standards Institute (CLSI). Different concentrations were prepared and the MICs were calculated by tube dilution method in triplicates. The MBCs were determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data generated were analyzed by ANOVA and Turkeys post hoc test using spss soft ware. The MIC of cockroach chitosan against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis, were found to be 240, 220, 0, 260, 240 and 0mg/ml while the MIC of cricket chitosan against the organisms were found to be 240,240,240,0, 220 and 280mg/ml. The MBC of chitosan from cockroach against the organisms were found to be 260, 260, 0, 280, 260 and 0 mg/ml while that of cricket were found to be 240, 240, 280, 0, 240 and 300 mg/ml. This study revealed that chitosan from P. Americana and A. domestica can be used as disinfectants in wounds, since they act against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis which are organisms commonly found in wound.

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)
DOI 10.11648/j.ijmb.20220704.14
Page(s) 177-182
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Chitosan, Antimicrobial Activity, Bacterial Isolates, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC)

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Cite This Article
  • APA Style

    Umar Zainab Garba, Abalaka Moses Enemaduku, Daniyan Safiya Yahaya, Babayi Hausatu. (2022). Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates. International Journal of Microbiology and Biotechnology, 7(4), 177-182. https://doi.org/10.11648/j.ijmb.20220704.14

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    ACS Style

    Umar Zainab Garba; Abalaka Moses Enemaduku; Daniyan Safiya Yahaya; Babayi Hausatu. Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates. Int. J. Microbiol. Biotechnol. 2022, 7(4), 177-182. doi: 10.11648/j.ijmb.20220704.14

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    AMA Style

    Umar Zainab Garba, Abalaka Moses Enemaduku, Daniyan Safiya Yahaya, Babayi Hausatu. Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates. Int J Microbiol Biotechnol. 2022;7(4):177-182. doi: 10.11648/j.ijmb.20220704.14

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  • @article{10.11648/j.ijmb.20220704.14,
      author = {Umar Zainab Garba and Abalaka Moses Enemaduku and Daniyan Safiya Yahaya and Babayi Hausatu},
      title = {Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {7},
      number = {4},
      pages = {177-182},
      doi = {10.11648/j.ijmb.20220704.14},
      url = {https://doi.org/10.11648/j.ijmb.20220704.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.14},
      abstract = {To determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of chitosan from cockroach and cricket against bacterial isolates, Staphylococccus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Streptococcus pyogenes, Bacillus subtilis and Pseudomonas aeruginosa. The antimicrobial efficacy of chitosan was determined by the standard method of Clinical and Laboratory Standards Institute (CLSI). Different concentrations were prepared and the MICs were calculated by tube dilution method in triplicates. The MBCs were determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data generated were analyzed by ANOVA and Turkeys post hoc test using spss soft ware. The MIC of cockroach chitosan against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis, were found to be 240, 220, 0, 260, 240 and 0mg/ml while the MIC of cricket chitosan against the organisms were found to be 240,240,240,0, 220 and 280mg/ml. The MBC of chitosan from cockroach against the organisms were found to be 260, 260, 0, 280, 260 and 0 mg/ml while that of cricket were found to be 240, 240, 280, 0, 240 and 300 mg/ml. This study revealed that chitosan from P. Americana and A. domestica can be used as disinfectants in wounds, since they act against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis which are organisms commonly found in wound.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates
    AU  - Umar Zainab Garba
    AU  - Abalaka Moses Enemaduku
    AU  - Daniyan Safiya Yahaya
    AU  - Babayi Hausatu
    Y1  - 2022/12/08
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijmb.20220704.14
    DO  - 10.11648/j.ijmb.20220704.14
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 177
    EP  - 182
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20220704.14
    AB  - To determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of chitosan from cockroach and cricket against bacterial isolates, Staphylococccus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Streptococcus pyogenes, Bacillus subtilis and Pseudomonas aeruginosa. The antimicrobial efficacy of chitosan was determined by the standard method of Clinical and Laboratory Standards Institute (CLSI). Different concentrations were prepared and the MICs were calculated by tube dilution method in triplicates. The MBCs were determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data generated were analyzed by ANOVA and Turkeys post hoc test using spss soft ware. The MIC of cockroach chitosan against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis, were found to be 240, 220, 0, 260, 240 and 0mg/ml while the MIC of cricket chitosan against the organisms were found to be 240,240,240,0, 220 and 280mg/ml. The MBC of chitosan from cockroach against the organisms were found to be 260, 260, 0, 280, 260 and 0 mg/ml while that of cricket were found to be 240, 240, 280, 0, 240 and 300 mg/ml. This study revealed that chitosan from P. Americana and A. domestica can be used as disinfectants in wounds, since they act against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis which are organisms commonly found in wound.
    VL  - 7
    IS  - 4
    ER  - 

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Author Information
  • Department of Microbiology, Federal University of Technology, Minna, Nigeria

  • Department of Microbiology, Federal University of Technology, Minna, Nigeria

  • Department of Microbiology, Federal University of Technology, Minna, Nigeria

  • Department of Microbiology, Federal University of Technology, Minna, Nigeria

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