Exploitation of PGPR Endophytic <i>Burkholderia</i> Isolates to Enhance Organic Agriculture

Sandanakirouchenane Aroumougame; Thirumangai Mannan Geetha; Muthu Thangaraju

doi:doi:10.11648/j.ajbio.20200803.12

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Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture

Sandanakirouchenane Aroumougame, Thirumangai Mannan Geetha, Muthu Thangaraju

Published in American Journal of BioScience (Volume 8, Issue 3)

Received: 18 September 2019 Accepted: 4 October 2019 Published: 28 May 2020

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Abstract

Although many bacterial species have been isolated from the rhizosphere of various crop plants, the recent discovery is Burkholderia sp., an endophytic bacterium. In this study, the Burkholderia isolates viz., RB₁ (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB₃ (Sugarcane Burkholderia 3) and BB₄ (Black gram Burkholderia 4) were enumerated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and black gram using N-free BAz (Burkholderia Azelaic acid) medium, in which black gram roots were observed higher population. Further, growth promoting activities of the Burkholderia isolates were examined, the maximum production of IAA and GA was noticed on the BB₄ as compared to other isolates and the cytokinin production was recorded more in isolates SB₃ followed by BB₄. Among the four isolates, maximum amount of salicylate type was noticed in RB₁ and catechol type was recorded higher in BB₄ which showed that these isolates were capable to produce Siderophore. The ACC deaminase activity of the isolates were exhibited, the BB₄ was recorded more followed by SB₃. Therefore, the endophytic Burkholderia isolates also the important contributor to the crop growth through secretion of growth promoting substances, production of siderophore and ACC deaminase activities may improve the Agriculture production.

Published in	American Journal of BioScience (Volume 8, Issue 3)
DOI	10.11648/j.ajbio.20200803.12
Page(s)	57-64
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Burkholderia Isolates, Endophytes, Indole Acetic Acid, Gibberellic Acid, Cytokinin, Siderophore, ACC Deaminase

References

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Sandanakirouchenane Aroumougame, Thirumangai Mannan Geetha, Muthu Thangaraju. (2020). Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture. American Journal of BioScience, 8(3), 57-64. https://doi.org/10.11648/j.ajbio.20200803.12

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ACS Style

Sandanakirouchenane Aroumougame; Thirumangai Mannan Geetha; Muthu Thangaraju. Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture. Am. J. BioScience 2020, 8(3), 57-64. doi: 10.11648/j.ajbio.20200803.12

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AMA Style

Sandanakirouchenane Aroumougame, Thirumangai Mannan Geetha, Muthu Thangaraju. Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture. Am J BioScience. 2020;8(3):57-64. doi: 10.11648/j.ajbio.20200803.12

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@article{10.11648/j.ajbio.20200803.12,
  author = {Sandanakirouchenane Aroumougame and Thirumangai Mannan Geetha and Muthu Thangaraju},
  title = {Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture},
  journal = {American Journal of BioScience},
  volume = {8},
  number = {3},
  pages = {57-64},
  doi = {10.11648/j.ajbio.20200803.12},
  url = {https://doi.org/10.11648/j.ajbio.20200803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20200803.12},
  abstract = {Although many bacterial species have been isolated from the rhizosphere of various crop plants, the recent discovery is Burkholderia sp., an endophytic bacterium. In this study, the Burkholderia isolates viz., RB1 (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB3 (Sugarcane Burkholderia 3) and BB4 (Black gram Burkholderia 4) were enumerated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and black gram using N-free BAz (Burkholderia Azelaic acid) medium, in which black gram roots were observed higher population. Further, growth promoting activities of the Burkholderia isolates were examined, the maximum production of IAA and GA was noticed on the BB4 as compared to other isolates and the cytokinin production was recorded more in isolates SB3 followed by BB4. Among the four isolates, maximum amount of salicylate type was noticed in RB1 and catechol type was recorded higher in BB4 which showed that these isolates were capable to produce Siderophore. The ACC deaminase activity of the isolates were exhibited, the BB4 was recorded more followed by SB3. Therefore, the endophytic Burkholderia isolates also the important contributor to the crop growth through secretion of growth promoting substances, production of siderophore and ACC deaminase activities may improve the Agriculture production.},
 year = {2020}
}

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TY  - JOUR
T1  - Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture
AU  - Sandanakirouchenane Aroumougame
AU  - Thirumangai Mannan Geetha
AU  - Muthu Thangaraju
Y1  - 2020/05/28
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbio.20200803.12
DO  - 10.11648/j.ajbio.20200803.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 57
EP  - 64
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20200803.12
AB  - Although many bacterial species have been isolated from the rhizosphere of various crop plants, the recent discovery is Burkholderia sp., an endophytic bacterium. In this study, the Burkholderia isolates viz., RB1 (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB3 (Sugarcane Burkholderia 3) and BB4 (Black gram Burkholderia 4) were enumerated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and black gram using N-free BAz (Burkholderia Azelaic acid) medium, in which black gram roots were observed higher population. Further, growth promoting activities of the Burkholderia isolates were examined, the maximum production of IAA and GA was noticed on the BB4 as compared to other isolates and the cytokinin production was recorded more in isolates SB3 followed by BB4. Among the four isolates, maximum amount of salicylate type was noticed in RB1 and catechol type was recorded higher in BB4 which showed that these isolates were capable to produce Siderophore. The ACC deaminase activity of the isolates were exhibited, the BB4 was recorded more followed by SB3. Therefore, the endophytic Burkholderia isolates also the important contributor to the crop growth through secretion of growth promoting substances, production of siderophore and ACC deaminase activities may improve the Agriculture production.
VL  - 8
IS  - 3
ER  -

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Author Information

Sandanakirouchenane Aroumougame

Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India
Thirumangai Mannan Geetha

Department of Agricultural Microbiology, Adhiparasakthi Agricultural College, Kalavai, India
Muthu Thangaraju

Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India

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Sandanakirouchenane Aroumougame, Thirumangai Mannan Geetha, Muthu Thangaraju. (2020). Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture. American Journal of BioScience, 8(3), 57-64. https://doi.org/10.11648/j.ajbio.20200803.12

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ACS Style

Sandanakirouchenane Aroumougame; Thirumangai Mannan Geetha; Muthu Thangaraju. Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture. Am. J. BioScience 2020, 8(3), 57-64. doi: 10.11648/j.ajbio.20200803.12

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AMA Style

Sandanakirouchenane Aroumougame, Thirumangai Mannan Geetha, Muthu Thangaraju. Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture. Am J BioScience. 2020;8(3):57-64. doi: 10.11648/j.ajbio.20200803.12

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@article{10.11648/j.ajbio.20200803.12,
  author = {Sandanakirouchenane Aroumougame and Thirumangai Mannan Geetha and Muthu Thangaraju},
  title = {Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture},
  journal = {American Journal of BioScience},
  volume = {8},
  number = {3},
  pages = {57-64},
  doi = {10.11648/j.ajbio.20200803.12},
  url = {https://doi.org/10.11648/j.ajbio.20200803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20200803.12},
  abstract = {Although many bacterial species have been isolated from the rhizosphere of various crop plants, the recent discovery is Burkholderia sp., an endophytic bacterium. In this study, the Burkholderia isolates viz., RB1 (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB3 (Sugarcane Burkholderia 3) and BB4 (Black gram Burkholderia 4) were enumerated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and black gram using N-free BAz (Burkholderia Azelaic acid) medium, in which black gram roots were observed higher population. Further, growth promoting activities of the Burkholderia isolates were examined, the maximum production of IAA and GA was noticed on the BB4 as compared to other isolates and the cytokinin production was recorded more in isolates SB3 followed by BB4. Among the four isolates, maximum amount of salicylate type was noticed in RB1 and catechol type was recorded higher in BB4 which showed that these isolates were capable to produce Siderophore. The ACC deaminase activity of the isolates were exhibited, the BB4 was recorded more followed by SB3. Therefore, the endophytic Burkholderia isolates also the important contributor to the crop growth through secretion of growth promoting substances, production of siderophore and ACC deaminase activities may improve the Agriculture production.},
 year = {2020}
}

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TY  - JOUR
T1  - Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture
AU  - Sandanakirouchenane Aroumougame
AU  - Thirumangai Mannan Geetha
AU  - Muthu Thangaraju
Y1  - 2020/05/28
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbio.20200803.12
DO  - 10.11648/j.ajbio.20200803.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 57
EP  - 64
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20200803.12
AB  - Although many bacterial species have been isolated from the rhizosphere of various crop plants, the recent discovery is Burkholderia sp., an endophytic bacterium. In this study, the Burkholderia isolates viz., RB1 (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB3 (Sugarcane Burkholderia 3) and BB4 (Black gram Burkholderia 4) were enumerated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and black gram using N-free BAz (Burkholderia Azelaic acid) medium, in which black gram roots were observed higher population. Further, growth promoting activities of the Burkholderia isolates were examined, the maximum production of IAA and GA was noticed on the BB4 as compared to other isolates and the cytokinin production was recorded more in isolates SB3 followed by BB4. Among the four isolates, maximum amount of salicylate type was noticed in RB1 and catechol type was recorded higher in BB4 which showed that these isolates were capable to produce Siderophore. The ACC deaminase activity of the isolates were exhibited, the BB4 was recorded more followed by SB3. Therefore, the endophytic Burkholderia isolates also the important contributor to the crop growth through secretion of growth promoting substances, production of siderophore and ACC deaminase activities may improve the Agriculture production.
VL  - 8
IS  - 3
ER  -

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