Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria
Gidado Rose Suniso Maxwell,
Etim Veronica Archibong,
Nweke Ogechi,
Iloh Andrew Chibuzor,
Isu Rosemary Nennaya,
Solomon Bamidele Ogbe
Issue:
Volume 4, Issue 5, September 2016
Pages:
58-63
Received:
25 April 2016
Accepted:
25 August 2016
Published:
12 September 2016
Abstract: Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.
Abstract: Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii...
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Responsiveness of Total Plasma Protein After Administration of Some Toxic Heavy Metals in an Indian Teleost (Clarias batrachus L.)
Abhijit Panigrahi,
Asok Dasmahapatra,
Ajit Kumar Medda
Issue:
Volume 4, Issue 5, September 2016
Pages:
64-73
Received:
9 June 2016
Accepted:
24 June 2016
Published:
15 November 2016
Abstract: Heavy metals of acetate salts of lead, zinc, copper and mercury cause serious toxic effects on protein biosynthesis. These metal salts reduce the plasma protein content. A dose-response relationship is found to occur in this blood parameter in the experimental fish in comparison to the control group of fishes. Both groups are fed. Higher dose of those chemical agents are much responsive to cause harmful effects on fish plasma protein amount. All the heavy metals toxicity except zinc toxicity are shown to be continued (reduced) upto day 42 but the decrease in the said parameter is more marked with zinc salt on day 7 only. The present investigation is an attempt to evaluate the relationship of this haematological parameter with the physiological status among the heavy metal polluted fishes during chronic treatment.
Abstract: Heavy metals of acetate salts of lead, zinc, copper and mercury cause serious toxic effects on protein biosynthesis. These metal salts reduce the plasma protein content. A dose-response relationship is found to occur in this blood parameter in the experimental fish in comparison to the control group of fishes. Both groups are fed. Higher dose of th...
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