Hsa-miR-106b-5p Negatively Regulates LEF1
Annada Anil Joshi,
Alka Vishwas Nerurkar,
Neelam Vishwanath Shirsat
Issue:
Volume 6, Issue 4, December 2018
Pages:
112-117
Received:
23 August 2018
Accepted:
10 September 2018
Published:
13 October 2018
DOI:
10.11648/j.crj.20180604.11
Downloads:
Views:
Abstract: Aberrant expression of the genes involved in Wnt signaling pathway, one of the most important developing pathways, is observed in many malignancies. Reports show that Wnt/β-catenin activation is critical for cancer development, angiogenesis, migration, and invasion. LEF1 belongs to the T cell Factor (TCF)/LEF family of transcription factors and plays the role of nuclear effector in the Wnt/β-catenin signaling pathway. LEF1 has central role as a transcription factor in the Wnt/β-catenin signaling pathway which makes it an ideal target for therapeutic treatment in dealing with cancer proliferation. It can act as an oncogene or a tumor suppressor in cellular context dependent manner. miRNAs are aberrantly expressed in cancers and can act as tumor suppressors or oncomirs depending upon the type of carcinomas. Studies show that miRNAs can be used as novel agents for targeted cancer therapy. miR-106b, which belong to miR-17-92 paralog cluster, is reported to be overexpressed in multiple tumor types including medulloblastomas, breast, colon, kidney, gastric, lung cancer and HCC. In this study we have demonstrated that over-expression of miR-106b-5p down-regulates the endogenous expression of LEF1 in HEK293FT cells, thereby affecting the expression of N-Myc, downstream gene of Wnt signaling. Therefore, our results suggest that miR-106b-5p plays a significant role in suppressing the carcinomas resulted due to the over-expression of LEF1 and/or activation of Wnt pathway and may prove to be a potential target for novel cancer therapy. It may helpful in developing therapeutic strategies for cancer treatments.
Abstract: Aberrant expression of the genes involved in Wnt signaling pathway, one of the most important developing pathways, is observed in many malignancies. Reports show that Wnt/β-catenin activation is critical for cancer development, angiogenesis, migration, and invasion. LEF1 belongs to the T cell Factor (TCF)/LEF family of transcription factors and pla...
Show More
Clinico-Epidemiological Study of Epithelial Ovarian Cancer Mansoura Experience
Soumaya Mohamed Eteba,
Maiy Abdul-Raheim Alshahat,
Fatma Mohamed Farouk Akl,
Nehal Kadry Mohammed
Issue:
Volume 6, Issue 4, December 2018
Pages:
118-125
Received:
15 November 2018
Accepted:
5 December 2018
Published:
18 December 2018
DOI:
10.11648/j.crj.20180604.12
Downloads:
Views:
Abstract: Background & objective: Ovarian cancer is the fifth most common cancer affecting women today. The aim of this retrospective study was to present the epidemiological, pathological, clinical characteristics of epithelial ovarian cancer patients treated at Clinical Oncology and Nuclear Medicine Department, Mansoura University Hospitals in the period between January 2009 and December 2013, with evaluation of progression free survival (PFS) overall survival (OS) and prognostic factors. Patients & methods: This is a retrospective study of epithelial ovarian carcinoma women treated at Clinical Oncology and Nuclear Medicine Department, Mansoura University Hospitals (in the period between January 2009 and December 2013). Data were analyzed after being collected from the patients' medical records. Clinical abstract sheets for all cases were designed. Results: The median age was 55 years (19 – 80 years), 58 patients (39.2%) were ECOG performance status (PS) 1, whereas, 50 patients (33.8%) were PS 0 at presentation. Ninety five patients (64.2%) were postmenopausal at presentation, serous cystadenocarcinoma was the predominant histopathologic type found in 94 patients (63.5%), followed by Endometrioid type (17.5%), 79 patients (53.4%) had stage III disease and 33 patients (22.3%) had stage IV disease. The majority of patients (99 patients 66.9%) had grade III tumors, 140 patients (94.6%) in the study underwent surgical treatment, optimal debulking was done in 85 patients (60.7%), while, suboptimal debulking was done in 55 patients (39.3%), the median overall survival was 49 months, while the median progression free survival was 18 months. Conclusion: the median age was 55 years old, serous carcinoma was the predominant histopathological type. Stage III and IV predominated. Most patients underwent initially surgery followed by adjuvant chemotherapy. The median overall survival & PFS were 49 & 18 months, respectively. Age, tumor histopathology, stage, grade and tumor residue after debulking surgery were significant independent prognostic factors for epithelial ovarian cancer.
Abstract: Background & objective: Ovarian cancer is the fifth most common cancer affecting women today. The aim of this retrospective study was to present the epidemiological, pathological, clinical characteristics of epithelial ovarian cancer patients treated at Clinical Oncology and Nuclear Medicine Department, Mansoura University Hospitals in the period b...
Show More
