Characterization of Extraceluller Chitinase Produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera
Jane Melita Keliat,
Dwi Suryanto,
Erman Munir
Issue:
Volume 2, Issue 5, October 2016
Pages:
24-27
Received:
13 September 2016
Accepted:
7 October 2016
Published:
8 November 2016
Abstract: The research was about characterization of extraceluller chitinase produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Precipitation of crude chitinase was performed with different levels of ammonium sulphate. Chitinase activity was determined colorimetrically by detecting the amount of N-acetylglucosamine (GlcNAc) released from colloidal chitin substrate with N-acetylglucosamine. Characterization of chitinase activity was measured at different pH, different temperature, Km and Vmax values. The optimum activity of chitinase was produced 4 days after incubation with chitinase activity of 0.0063 U/ml. Optimum chitinase activity was higher after 50% ammonium sulphate precipitation with activity of 0.0087 U/ml followed by decreasing in activity supernatant with activity of 0.0012 U/ml. Chitinase activity increased at pH 6 and at 60°C by 0.05066 U/ml with specific activity 3.8113 U/mg after dialysis. Km and Vmax values were 0.321 mg/ml and 71.429 µg, respectively.
Abstract: The research was about characterization of extraceluller chitinase produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Precipitation of crude chitinase was performed with different levels of ammonium sulphate. Chitinase activity was determined colorimetrically by detecting the amount of N-acetylglucosamine (GlcNAc) releas...
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